产地：MBI 

货号：EN0141

Applications

High-throughput methods for mutation research (3, 4).

Studies in mutagenesis and DNA repair.

Mismatch cleavage.

Genotyping.

Description

Endonuclease V, T.maritima (Endo V), is a 3'-endonuclease involved in DNA repair which initiates removal of deaminated bases from damaged DNA, including uracil, hypoxanthine and xanthine, see Fig.1.

Endonuclease V is also active toward abasic sites and urea sites, base pair mismatches, flap and pseudo Y structures, and small insertions/deletions in DNA molecules. The cleavage site generated by Endonuclease V is at the second phosphodiester bond 3 to a lesion. When the enzyme is in excess, the primary nicked products experience a second nicking event on the complementary strand, leading to a double-stranded break. At low concentrations, however, Endonuclease V first nicks a DNA strand at the lesions located closer to the 5-end of DNA molecule. Single-stranded DNA is cleaved with much lower efficiency. Mg2+ or Mn2+ ions are required for enzyme activity (1, 2, 3).