Removal of mRNA prior to synthesis of second strand cDNA (1).
RT-PCR and qRT-PCR: removal of RNA after first strand cDNA synthesis.
Removal of the poly(A) sequences of mRNA after hybridization with oligo(dT) (2).
Site-specific cleavage of RNA (3).
Studies of in vitro polyadenylation reaction products (4).
Description
Ribonuclease H (RNase H) specifically degrades the RNA strand in RNA-DNA hybrids, see Fig.1. It does not hydrolyze the phosphodiester bonds within single-stranded and double-stranded DNA and RNA.
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