Removal of RNA from recombinant protein preparations.
Ribonuclease protection assays. Used in conjunction with RNase T1 (3).
Mapping single-base mutations in DNA or RNA (5, 6).
Description
RNase A, DNase and protease-free is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues, see Fig.1.
It cleaves the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide. The resulting 2', 3'-cyclic phosphate is hydrolyzed to the corresponding 3'-nucleoside phosphate (1, 2).
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