Removal of single-stranded DNA containing a 3'-hydroxyl terminus from nucleic acid mixtures.
Assay for the presence of single-stranded DNA with a 3'-hydroxyl terminus (4).
Description
Exonuclease I (ExoI) degrades single-stranded DNA in a 3'=>5' direction, releasing deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaving 5'-terminal dinucleotides intact, see Fig.1.
It does not cleave DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups (1).
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