Acetyl CoA Carboxylase 1(ACC1)(ACACA)antibody
Cat.#: 1768-1
Rabbit Monoclonal Antibody

Clone ID: EP687Y
Swiss Prot: Q13085
Mol Weight: 265kDa
Size: 100ul

Description
Acetyl-CoA carboxylase 1 (ACC1) is a biotin dependent lipogenic enzyme that is highly expressed during adipogenesis. ACC1 catalyzes acetyl-CoA carboxylation, producing malonyl-CoA, a metabolite involved in energy homeostasis regulation. Malonyl-CoA is a two carbon donor in the synthesis of long-chain fatty acids and the elongation of fatty acids found in the cystol (1). ACC1 is regulated short-term by citrate, CoA, and palmitoyl-CoA through allosteric interactions. Nutrients and hormones can be both short-term (inducing reversible phosphorylations by such as AMPK) and long-term (transcription level regulation) regulators of ACC1 (2). Highly expressed in lipogenic tissues, ACC1 is found in liver, adipose, and lactating mammary gland (3). ACC1 has been implicated as a target in the development of anti-obesity drugs (4).

Recommended Applications
WB, IHC

Applications and Recommended Dilution Factors
WB: 1:500 - 2000
IHC: 1:50 - 100

Species Reactivity
Human, Mouse, Rat

For Mouse, this antibody is recommended for WB only
Rat cross reactivity tested by western blot only

Products Data 

 

A. Western blot analysis on A431 cell lysate using anti-Acetyl-CoA carboxylase RabMAb (cat. # 1768-1), dilution 1:2000
B. Immunohistochemical analysis of paraffin-embedded human liver using anti-AcetylCoenzyme A RabMAb (cat. # 1768-1).

Specificity
A synthetic peptide corresponding to residues in the human Acetyl-CoA carboxylase 1 was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ACACA antibody, ACAC antibody, ACC antibody, ACC1 antibody, ACCA antibody, Biotin carboxylase antibody, ACC-alpha antibody

Description References
1. Jianqiang M, et al. PNAS 100:7515-7520, 2003.
2. Girad J, et al. The FASEB Journal 8:36-42, 1994.
3. Wakil SJ, et al. Annu Rev Biochem 52:537-539
4. Abu-Elheiga L, et al. Science 291: 2613-2616. 2001.

Product References
1. Wong GW, Krawczyk SA, Kitidis-Mitrokostas C, et al, Identification and characterization of CTRP9, a novel secreted glycoprotein, from adipose tissue that reduces serum glucose in mice and forms heterotrimers with adiponectin ,FASEB J 23 (2009) 241-258
[Application: WB]
2. Ota S, Horigome K, Ishii T, et al, Metformin suppresses glucose-6-phosphatase expression by a complex I inhibition and AMPK activation-independent mechanism ,Biochem Biophys Res Commun 388 (2009) 311-316
[Application: WB]
3. Yi F, Chen QZ, Jin S, et al, Mechanism of Homocysteine-Induced Rac1/ NADPH Oxidase Activation in Mesangial Cells: Role of Guanine Nucleotide Exchange Factor Vav2 ,Cell Physiol Biochem 20 (2007) 909-918
[Application: WB]