Adiponectin(ADIPOQ)antibody
Cat.#: 2548-1
Rabbit Monoclonal Antibody
Clone ID: EPR3217
Swiss Prot: Q15848
Mol Weight: 28/56/65 kDa (28 kDa monomer/56 kDa dimer, 65kDa
Size: 100ul

Description
Adiponectin is a multimeric adipocyte-specific secretory protein that regulates energy homeostasis, lipid metabolism, and insulin sensitivity with direct anti-diabetic, anti-atherogenic and anti-inflammatory properties. Adiponectin predominantly inhibits proliferation of myelomonocytic lineage cells and is an important negative regulator in hematopoiesis and immune systems (1, 2). Adiponectin is reported to possibly function as an endogenous factor involved in lipid catabolism and storage or whole body metabolism (3). It is decreased in obesity and has been demonstrated to inhibit tumor necrosis factor-alpha (TNF-alpha)-induced expression of endothelial adhesion molecules. Adiponectin modulates the inflammatory response of endothelial cells, possibly through cross talk between cAMP-PKA and NF-kappaB signaling pathways (4). Adiponectin forms trimers, hexamers and 12- to 18-mers. The trimers are low molecular weight complexes (LMW). Several trimers can associate to form disulfide-linked hexamers (middle molecular weight complexes (MMW)) and higher molecular weight (HMW) complexes (5).

Recommended Applications
WB, ICC

Applications and Recommended Dilution Factors
WB: 1:2,000 - 5,000
ICC: 1:100 - 250

Species Reactivity
Human

Cross reactivity determined by western blot only.

Products Data 

 

A.Western blot analysis on (A) fetal fat and (B) human plasma lysates using anti-Adiponectin RabMAb (cat. #2548-1), dilution 1:5000.
B. Western blot analysis on human plasma lysates treated with (A) Beta-ME and heat, (B) heat only, (C) Beta-ME only, and (D) no treatment using anti-Adiponectin RabMAb (cat. #2548-1).

Specificity
A synthetic peptide corresponding to residues on human Adiponectin was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ADIPOQ antibody, ACDC antibody, ACRP30 antibody, ADIPQTL1 antibody, ADPN antibody, APM-1 antibody, APM1 antibody, GBP28 antibody, adiponectin antibody, Adiponectin antibody, Gelatin-binding protein antibody

Description References
1. Yokota T, et al. Blood 96(5):1723-32, 2000
2. Richards AA, et al. Mol Endocrinol. 20(7):1673-87, 2006
3. Nakano Y, et al. J Biochem. 120(4):803-12, 1996
4. Ouchi N, et al. Circulation. 102(11):1296-301, 2000
5. The UniProt Consortium. The Universal Protein Resource (UniProt). Nucleic Acids Res. 36:D190-D195 (2008)