AE1(SLC4A1)antibody
Cat.#: 3637-1
Rabbit Monoclonal Antibody
Clone ID: EPR1426
Swiss Prot: P02730
Mol Weight: 100kDa
Size: 100ul

Description
The AE1 (anion exchanger, band 3) protein is expressed in erythrocytes and in the A-type intercalated cells of the kidney distal collecting tubule. In both cell types it mediates the electroneutral transport of chloride and bicarbonate ions across the lipid bilayer, and, in erythrocytes (1). AE1 is phosphorylated on Tyr8and Tyr21 by SYK. It has been suggested that, upon phosphorylation by p72syk, Tyr8, and Tyr21 act as docking sites for the SH2 domain of Lyn, which subsequently phosphorylates AE1 at additional secondary sites (2).

Recommended Applications
WB, IHC

Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1:100 - 250

Species Reactivity
Human

Cross reactivity determined by western blot only

Products Data

 

Western blot analysis on Red blood cell lysate using anti-AE1 RabMAb (cat. #3637-1). 
Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-AE1 (cat. #3637-1). 
Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-AE1 (cat. #3637-1). 

Specificity
A synthetic peptide corresponding to residues in human AE1 was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
SLC4A1 antibody, AE1 antibody, BND3 antibody, CD233 antibody, DI antibody, EMPB3 antibody, EPB3 antibody, FR antibody, MGC116750 antibody, MGC116753 antibody, MGC126619 antibody, MGC126623 antibody, RTA1A antibody, SW antibody, WD antibody, WD1 antibody, WR antibody, Band 3 anion transport protein antibody, Solute carrier family 4 member 1 antibody

Description References
1. Sahr KE, et al. Genomics 24(3):491-501, 1994
2. Brunati AM, et al. Blood 96(4):1550-7, 2000