Alpha-1 Catenin(CTNNA1)antibody
Cat.#: 2028-1
Rabbit Monoclonal Antibody

Clone ID: EP1793Y
Swiss Prot: P35221
Mol Weight: 100kDa
Size: 100ul

Description
Cadherin and catenin compose cell adhesion complex and are indispensable for tight cell-cell adhesion. Dysfunction of this adhesion complex causes dissociation of cancer cells from primary tumor nodules, thus possibly contributing to cancer invasion and metastasis (1). At least three proteins (alpha, beta, and gamma catenin) comprise the cytoplasmic domain of the cadherin cell-cell adhesion complex. Data, with the reported structure of other catenin genes, suggest that vinculin and alpha-catenin generate a superfamily of proteins mediating membrane-cytoskeletal associations (2). Presenilin-1 (PS1) overexpression in human kidney cells enhances cell-cell adhesion and data show that PS1 incorporates into the cadherin/catenin adhesion system and regulates cell-cell adhesion. PS1 concentrates at intercellular contacts in epithelial tissue; in brain, it forms complexes with both E- and N-cadherin and concentrates at synaptic adhesions (3).

Recommended Applications
WB, IHC, ICC, IP, FC

Applications and Recommended Dilution Factors
WB: 1:10,000 - 50,000
IHC: 1:100 - 250
ICC: 1:100 - 250
IP: 1:50
FC: 1:50

Species Reactivity
Human, Mouse, Rat

Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

Products Data 

 

A. Western blot analysis on HeLa cell lysate using anti-Alpha-1 Catenin RabMAb (cat. # 2028-1), dilution 1:50,000.
B. Immunohistochemical staining of paraffin-embedded human breast carcinoma using anti-Alpha-1 Catenin RabMAb (cat. #2028-1).

Specificity
A synthetic peptide corresponding to residues near the N-terminus of human α-1 catenin was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
CTNNA1 antibody, CAP102 antibody, FLJ36832 antibody, FLJ52416 antibody, Catenin alpha-1 antibody, Alpha E-catenin antibody, Cadherin-associated protein antibody, Renal carcinoma antigen NY-REN-13 antibody

Description References
1. Oda T, et al. Biochem Biophys 193(3):897-904, 1993
2. Rimm DL, et al. Biochem Biophys 203(3):1691-9, 1994
3. Georgakopoulos A, et al. Mol Cell 4(6):893-902, 1999

Product References
1. Lien WH, Gelfand VI, Vasioukhin V, α– E-catenin binds to dynamitin and regulates dynactin-mediated intracellular traffic ,J. Cell Biol. 183 (2008) 989 - 997
[Application: IP]