ALPL antibody
Cat.#: 3162-1
Rabbit Monoclonal Antibody
Clone ID: EPR4477
Swiss Prot: P05186
Mol Weight: 80kDa
Size: 100ul

Description
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. ALPL is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms (1).

Recommended Applications
WB, IHC, ICC, IP

Applications and Recommended Dilution Factors
WB: 1:10,000 - 50,000
IHC: 1:250 - 500
ICC: 1:100 - 500
IP: 1:10 - 100

Species Reactivity
Human, Mouse, Rat

Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

Products Data  

 

Western blot analysis on (A) SAOS-2, (B) HepG2, (C) HeLa, and (D) JAR cell lysates using anti-ALPL RabMAb (cat. #3162-1).
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ALPL RabMAb (cat. #3162-1).
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ALPL RabMAb (cat. #3162-1).
 

Immunofluorescent staining of HeLa cells using anti-ALPL RabMAb (cat. #3162-1).

Specificity
A synthetic peptide corresponding to residues in human ALPL was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ALPL antibody, AP-TNAP antibody, APTNAP antibody, FLJ40094 antibody, FLJ93059 antibody, HOPS antibody, MGC161443 antibody, MGC167935 antibody, TNAP antibody, TNSALP antibody, Alkaline phosphatase, tissue-nonspecific isozyme antibody, Alkaline phosphatase liver/bone/kidney isozyme antibody

Description References
1. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1; 33: D54-8.