Androgen Receptor(AR)antibody
Cat.#: 3184-1
Rabbit Monoclonal Antibody
Clone ID: ER179(2)
Swiss Prot: P10275
Mol Weight: 110kDa
Size: 100ul

Description
Androgen receptor (AR) is a member of the steroid receptor superfamily that may require coactivators for proper or maximal transactivation. (1). The androgen receptor (AR) is essential for the growth of prostate cancer cells. It has been reported that tyrosine phosphorylation of AR is induced by growth factors and elevated in hormone-refractory prostate tumors. Data suggest that growth factors and their downstream tyrosine kinases, which are elevated during hormone-ablation therapy, can induce tyrosine phosphorylation of AR and such modification may be important for prostate tumor growth under androgen-depleted conditions (2). Cellular signaling occurs following androgen binding to the AR and translocation to the nucleus. This activated complex associates with androgen-responsive elements contained in the DNA sequence of target genes, affecting the transcriptional activity of these genes. Mutations may result in changes in the function or expression of AR protein, or of growth factors and their receptors under the influence of AR coactivators, and AR amplification could lead to activation of the receptor by reduced levels of androgens (3).

Recommended Applications
WB, IHC, ICC, IP

Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1:250 - 500
ICC: 1:100 - 250
IP: 1:10 - 100

Species Reactivity
Human

Species cross-reactivity is based on WB analysis

Products Data  

 

Western blot analysis on (A) T47-D and (B) LnCaP cell lysates using anti-Androgen Receptor RabMAb (cat. #3184-1).
Immunohistochemical analysis of paraffin-embedded human prostatic adenocarcinoma tissue using anti-Androgen Receptor RabMAb (cat. #3184-1).
Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Androgen Receptor RabMAb (cat. #3184-1).
 

Immunofluorescent staining of LnCap cells using anti-Androgen Receptor RabMAb (cat. #3184-1).

Specificity
A synthetic peptide corresponding to residues in the N-terminus of human Androgen Receptor was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
AR antibody, AIS antibody, DHTR antibody, HUMARA antibody, HYSP1 antibody, KD antibody, NR3C4 antibody, SBMA antibody, SMAX1 antibody, TFM antibody, Androgen receptor antibody, Dihydrotestosterone receptor antibody, Nuclear receptor subfamily 3 group C member 4 antibody

Description References
1. Callewaert L, et al. Biochem Biophys Res Commun 306(1) 46-52, 2003.
2. Qiu Y, et al. Cancer Cell Vol 10:309-319, 2006.
3. Hamdy F, et al. Nature Clinical Practice Urology 2:368-399 2005.