ARF1 antibody
Cat.#: 3322-1
Rabbit Monoclonal Antibody
Clone ID: EPR443
Swiss Prot: P84077
Mol Weight: 21kDa
Size: 100ul
Description
ARF1 (small GTP-binding ADP-ribosylation factor 1) is a member of the ARF family, which contains five other ARF proteins (1). ARF1 functions in various membrane trafficking events in the ER-Golgi system and in the maintenance of organelle structure. Inactive ARF1 (ARF1-GDP) localizes in the cytoplasm, while the active form (ARF1-GTP) localizes in the membrane (2-3). Active ARF1 is also localized to the Golgi complex by regulating the assembly of coat proteins onto membranes and mediating traffic in the ER-Golgi. ARF1 activates phospholipase D and phosphatidylinositol 4-phosphate 5-kinase and recruits COPI to membranes (4-5).
Recommended Applications
WB, IHC, ICC
Applications and Recommended Dilution Factors
WB: 1:1,000-10,000
IHC: 1:100 - 250
ICC: 1:50 - 100
Species Reactivity
Human, Mouse, Rat
Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only
Products Data
Specificity
A synthetic peptide corresponding to residues in human ARF1 was used as an immunogen.
Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Alternative Names
ARF1 antibody, - antibody, ADP-ribosylation factor 1 antibody, antibody
Description References
1. Bobak et al. Proc. Nat. Acad. Sci. 86: 6101-6105, 1989.
2. Serafini, T., Orci, L., Amherdt, M., Brunner, M., Kahn, R. A., and Rothman, J. E. (1991) Cell 67, 239
3. Yang, C. Z., Heimberg, H., D'Souza-Schorey, C., Mueckler, M. M., and Stahl, P. D. (1998) J. Biol. Chem. 273, 4006
4. Bonifacino, J.S., and J. Lippincott-Schwartz. 2003. Nat. Rev. Mol. Cell Biol. 4:409
5. Nie, Z., D.S. Hirsch, and P.A. Randazzo. 2003. Arf and its many interactors. Curr. Opin. Cell Biol. 15:396