ATM Phospho(pS1981)antibody
Cat.#: 2152-1
Rabbit Monoclonal Antibody
Clone ID: EP1890Y
Swiss Prot: Q13315
Mol Weight: 370kDa
Size: 100ul

Description
ATM is a nuclear phosphoprotein involved in Ataxia-telangiectasia (A-T) an autosomal recessive disorder characterized by cerebellar ataxia, immune deficiencies, increased cancer predisposition, chromosomal instability and radiation sensitivity (1-2). This large protein (370 kDa) is involved in genome stability, cellular responses to DNA damage and cell cycle control. Autophosphorylation at serine 1981 on ATM is required during ATM kinase activation initiated by double strand breaks in the genome. After serine 1981 autophosphorylation, ATM phosphorylates downstream proteins (e.g. p53, Chk2), leading to cell cycle arrest and DNA repair (3). More than 100 mutations have been identified so far and are expected to inactivate the ATM protein by truncation or large deletions (4). Defects in ATM contribute to various B-cell and T-cell leukemias (5).

Recommended Applications
WB, IHC, ICC, IP

Applications and Recommended Dilution Factors
WB: 1:1000 - 10,000
IHC: 1:100 - 250
ICC: 1:100 - 250
IP: 1:40

Species Reactivity
Human

Cross reactivity determined by western blot only.

Products Data 

 

A. Western blot analysis on HEK293 cell lysates using anti-ATM Phospho (pS1981) RabMAb (cat # 2152-1), dilution at 1:10,000. Cells were either (A) untreated or (B) treated with doxorubicin.
B. Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using anti-ATM Phospho (pS1981) RabMAb (cat# 2152-1).

Specificity
A synthetic peptide corresponding to residues surrounding serine 1981 of human ATM was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ATM antibody, AT1 antibody, ATA antibody, ATC antibody, ATD antibody, ATDC antibody, ATE antibody, DKFZp781A0353 antibody, MGC74674 antibody, TEL1 antibody, TELO1 antibody, Serine-protein kinase ATM antibody, Ataxia telangiectasia mutated antibody

Description References
1. Harnden, D.G.; Int. J. Radiat. Biol. 66:S13-S19, 1994.
2. Lavin MF, Shiloh,Y. Ann. Rev. Imm. 15:177-202, 1997
3. Goldstine JV, et al. DNA Repair, 5:432-443, 2006.
4. Savitsky K et al. Hum. Mol. Gen. 4:2025-2032, 1995.
5. Stankovic T.et al. Lancet 353:26-29., 1999.

Product References
1. Momcilovic O, Choi S, Varum S, et al, Ionizing Radiation Induces Ataxia Telangiectasia Mutated-Dependent Checkpoint Signaling and G2 But Not G1 Cell Cycle Arrest in Pluripotent Human Embryonic Stem Cells ,Stem Cells 27 (2009) 1822 -1835
2. Amé JC, Fouquerel E, Gauthier LR, et al, Radiation-induced mitotic catastrophe in PARG-deficient cells ,J. Cell Sci. 122 (2009) 1990 - 2002
3. Togano T, Sasaki M, Watanabe M, et al, Induction of oncogene addiction shift to NF-κB by camptothecin in solid tumor cells ,Biochem Biophys Res Commun 390 (2009) 60-64
[Application: WB]
4. Goff JP, Shields DS, Seki M, et al, Lack of DNA Polymerase θ (POLQ) Radiosensitizes Bone Marrow Stromal Cells In Vitro and Increases Reticulocyte Micronuclei after Total-Body Irradiation ,Radiat Res 172 (2009) 165-174
[Application: IP]
5. Beyrouthy MJ, Garner KM, Hever MP, et al, High DNA Methyltransferase 3B Expression Mediates 5-Aza-Deoxycytidine Hypersensitivity in Testicular Germ Cell Tumors ,Cancer Res. 69 (2009) 9360-9366
6. Noon AT, Shibata A, Rief N, et al, 53BP1-dependent robust localized KAP‑1 phosphorylation is essential for heterochromatic DNA double-strand break repair ,Nat Cell Biol 12 (2010) 177-184
[Application: ICC]
7. Pires IM, Bencokova Z, Milani M, et al, Effects of Acute versus Chronic Hypoxia on DNA Damage Responses and Genomic Instability ,Cancer Res 70 (2010) 925-935
8. Gagou ME, Zuazua-Villar P, Meuth M, Enhanced H2AX Phosphorylation, DNA Replication Fork Arrest, and Cell Death in the Absence of Chk1 ,Mol Biol Cell 21 (2010) 739-752
9. Williamson CT, Muzik H, Turhan AG, et al, ATM Deficiency Sensitizes Mantle Cell Lymphoma Cells to Poly(ADP-Ribose) Polymerase-1 Inhibitors ,Mol Cancer Ther 9 (2010) 347-357
10. Kobayashi J, Kato A, Ota Y, et al, Bisbenzamidine derivative, pentamidine represses DNA damage response through inhibition of histone H2A acetylation ,Mol Cancer 9 (201) :34
[Application: ICC]
11. White JS, Choi S, Bakkenist CJ, Transient ATM Kinase Inhibition Disrupts DNA Damage–Induced Sister Chromatid Exchange ,Sci Signal 3 (2010) ra44
[Application: WB]
12. Boichuk S, Hu L, Hein J, et al, Multiple DNA Damage Signaling and Repair Pathways Deregulated by Simian Virus 40 Large T Antigen ,J Virol 84 (2010) 8007-8020
13. Bensimon A, Schmidt A, Ziv Y, et al, ATM-Dependent and -Independent Dynamics of the Nuclear Phosphoproteome After DNA Damage ,Sci Signal 3 (2010) rs3
14. Huang SM, Lu KT, Wang YC, ATM/ATR and SMAD3 Pathways Contribute to 3-Indole-induced G1 Arrest in Cancer Cells and Xenograft Models ,Anticancer Res 31 (2011) 203-208
[Application: WB]