Aurora-B(N-term)(AURKB)antibody
Cat.#: 1788-1
Rabbit Monoclonal Antibody
Clone ID: EP1009Y
Swiss Prot: Q96GD4
Mol Weight: 39kDa
Size: 100ul

Description
Aurora-B is a member of a novel family of serine/threonine kinases that have been identified as key regulators of the mitotic cell division process. Aurora-B is known to be involved in the regulation of centrosome function, bipolar spindle assembly and chromosome segregation. Aurora kinases are localized at the centrosomes of interphase cells, at the poles of the bipolar spindle and in the midbody of the mitotic apparatus. Substrates identified for the Aurora-B kinases include a kinesin-like motor protein, spindle apparatus proteins, histone H3 protein, kinetochore protein and the tumor suppressor protein p53 (1). Overexpression of aurora B produced multinuclearity and induced aggressive metastasis, suggesting that overexpressed aurora B has multiple functions in cancer development. Identification of Aurora kinases as RasGAP Src homology 3 domain binding protein, also implicates these kinases as potential effectors in the Ras pathway relevant to oncogenesis (2).

Recommended Applications
WB, IHC, ICC, IP

Applications and Recommended Dilution Factors
WB: 1:1,000-10,000
IHC: 1:250-500
ICC: 1:100-250
IP: 1:50

Species Reactivity
Human

Cross reactivity determined by western blot only.

Products Data 

 

A. Western blot analysis on HeLa cell lysate using anti-Aurora-B RabMAb (cat. # 1788-1), dilution 1:50,000.
B. Immunohistochemical staining of paraffin-embedded human Hodgking lymphoma using anti-Aurora-B RabMAb (cat.#1788-1).

Specificity
A synthetic peptide corresponding to residues near the N-terminus of human Aurora-B was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
AURKB antibody, AIK2 antibody, AIM-1 antibody, AIM1 antibody, ARK2 antibody, AurB antibody, IPL1 antibody, STK12 antibody, STK5 antibody, aurkb-sv1 antibody, aurkb-sv2 antibody, Serine/threonine-protein kinase 12 antibody, STK-1 antibody, Serine/threonine-protein kinase aurora-B antibody

Description References
1. Katayama H, et al. Cancer Metastasis Rev. 22(4): 451-64, 2003
2. Abdulla A, et al. Cancer Cell International 5(31), 2005

Product References
1. Temme A, Geiger KD, Wiedemuth R, et al, Giant Cell Glioblastoma Is Associated With Altered Aurora B Expression and Concomitant p53 Mutation ,J Neuropathol Exp Neurol 69 (2010 632-642
[Application: IHC]
2. Ikezoe T, Takeuchi T, Yang J, et al, Analysis of Aurora B kinase in non-Hodgkin lymphoma ,Lab Invest 89 (2009) 1364-1373
[Application: IHC]
3. Fiskus W, Wang Y, Joshi R, et al, Co-treatment with vorinostat enhances activity of MK-0457 (VX-680) against acute and chronic myelogenous leukemia cells ,Clin Cancer Res. 14 (2008) 6106-6115
[Application: WB]