AIF antibody
Cat.#: S2715
Rabbit Polyclonal Antibody
Clone ID: polyclonal
Swiss Prot: O95831
Mol Weight: 66.3kDa
Size: 100ug

Description
This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Several alternative transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq]

Recommended Applications
WB, IHC

Applications and Recommended Dilution Factors
WB: 1:500-1:3000
IHC: 1:100-1:250

Species Reactivity
Human

Other species have not been tested

Products Data 

 

Sample (30ug of whole cell lysate)
A: H1299
B: Hep G2
7.5% SDS PAGE
diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using AIF antibody at 1:100 dilution.

Specificity
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 78 and 296 of AIF

Storage Condition and Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Purified by antigen-affinity chromatography.

Alternative Names
AIFM1 antibody, AIF antibody, COXPD6 antibody, MGC111425 antibody, PDCD8 antibody, Apoptosis-inducing factor 1, mitochondrial antibody, Programmed cell death protein 8 antibody