Catalogue Number | ANT-102 |
Synonyms | T-cell growth factor (TCGF), Aldesleukin, Lymphokine, IL-2. |
Type | Mouse Anti Human Monoclonal. |
Introduction | IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. |
Clone | YNRhIL2. |
Immunogen | r.Human IL-2. |
Ig Subclass | Mouse IgG2a. |
Purification Method | Ion Exchange. |
Protein concentration | 1mg/ml in PBS (after reconstitution). |
Shipping Conditions | Antibody is shipped lyophilized at ambient temperature. |
Storage Procedures | In lyophilized form, for long periods, store at 4°C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20°C. |
Reconstitution | Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. |
Stability / Shelf Life | Two years lyophilized, one month in solution at 4°C. |
Titer By direct ELISA | 1:10,000 dilution will yield 0.4 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories). 1:50 dilution will block 70-80% of CTLL proliferative response to 50 units of r.human IL-2. |
Applications | Direct ELISA, Western Blot, Immuneprecipitation, immunohistochemistry, blocking T cell poroliferation. |
Cross reactivity | A certain degree of functional cross reactivity with murine IL-2 was reported by some investigators. |
Usage | Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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