Catalogue Number | ANT-114 |
Synonyms | NKSF2, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor 40 kDa subunit (CLMF p40), TSF, Edodekin-alpha, IL-12 p40, IL-12B, IL-12 subunit p40, NK cell stimulatory factor chain 2. |
Type | Rat Anti Mouse Monoclonal. |
Introduction | Active IL-12 is a p70 disulphide-linked dimer composed of p35 and p40 subunits. The protein is a pleiotropic cytokine produced primarily by antigen presenting cells and has multiple effects on T lymphocytes and natural killer cells in terms of stimulating cytotoxicity, proliferation, production of other cytokines and Th1 subset differentiation. |
Clone | NYRmIL-12p40. |
Immunogen | r.Murine IL-12 p40. |
Ig Subclass | Rat IgG2a. |
Purification Method | Ion exchange. |
Protein concentration | 1mg/ml in PBS (after reconstitution). |
Shipping Conditions | Antibody is shipped lyophilized at ambient temperature. |
Storage Procedures | In lyophilized form, for long periods, store at 4oC in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20oC. |
Reconstitution | Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf LifeTwo years lyophilized, one month in solution at 4oC. |
Applications | Direct ELISA, Western Blot, Immuneprecipitation. |
Titer | By direct ELISA, 1:5,000 dilution will yield 0.5 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories). |
Usage | Prospec's products are furnished for LABORATORY ESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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