alpha Tubulin antibody
Cat.#: S3251
Rabbit Polyclonal Antibody
Clone ID: polyclonal
Swiss Prot: Q71U36
Mol Weight: 50.14kDa
Size: 100ul

Description
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. [provided by RefSeq]

Recommended Applications
WB

Applications and Recommended Dilution Factors
WB: 1:500-1:3000

Species Reactivity
Human

Other species have not been tested

Products Data

Sample (30ug of whole cell lysate)
A: 293T
B: A431
C: JurKat
D: Raji
10% SDS PAGE
diluted at 1:1000

Specificity
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 227 and 450 of alpha Tubulin

Storage Condition and Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Purified by antigen-affinity chromatography.

Alternative Names
TUBA1A antibody, B-ALPHA-1 antibody, FLJ25113 antibody, LIS3 antibody, TUBA3 antibody, Tubulin alpha-1A chain antibody, Alpha-tubulin 3 antibody, Tubulin B-alpha-1 antibody, Tubulin alpha-3 chain antibody