AChE antibody
Cat.#: S0902
Rabbit Polyclonal Antibody
Clone ID: polyclonal
Swiss Prot: P22303
Mol Weight: 67.81kDa
Size: 100ug

Description
Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. [provided by RefSeq]

Recommended Applications
WB, ICC

Applications and Recommended Dilution Factors
WB: 1:500-1:3000
ICC:

Species Reactivity
Human
Other species have not been tested

Products Data

Sample (30ug of whole cell lysate)
A: Raji
7.5% SDS PAGE
diluted at 1:1000

Specificity
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 406 and 610 of Human ACHE

Storage Condition and Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Purified by antigen-affinity chromatography.

Alternative Names
ACHE antibody, ARACHE antibody, N-ACHE antibody, YT antibody, Acetylcholinesterase antibody, antibody