ADAM17 Antibody(N-term)
Cat.#: T0330
Rabbit Polyclonal Antibody
Clone ID: polyclonal
Swiss Prot: P78536
Mol Weight: 93kDa
Size: 100ug

Description
ADAM17 is a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. ADAM17 functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway.

Recommended Applications
WB, FC, ELISA

Applications and Recommended Dilution Factors
WB: 1:50~100
FC: 1:10~50
ELISA: 1:1,000

Species Reactivity
Human

Products Data 

 

Western blot analysis of ADAM17 Antibody (N-term)in CEM cell line lysates (35ug/lane). ADAM17 (arrow) was detected using the purified Pab (1:60 dilution).
Flow cytometric analysis of CEM cells using ADAM17 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specificity
This ADAM17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 202~232 amino acids from the N-terminal region of human ADAM17.

Storage Condition and Buffer
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. Then the antibody is purified by peptide affinity purification.

Alternative Names
ADAM17 antibody, ADAM18 antibody, CD156B antibody, CSVP antibody, MGC71942 antibody, TACE antibody, Disintegrin and metalloproteinase domain-containing protein 17 antibody, Snake venom-like protease antibody, TNF-alpha convertase antibody, TNF-alpha-converting enzyme antibody

Description References
Tellier,E., J. Cell. Physiol. 214 (3), 687-693 (2008)
Takamune,Y., Biochem. Biophys. Res. Commun. 365 (2), 393-398 (2008)