Ape 1(APEX1)antibody
Cat.#: 2851-1
Rabbit Monoclonal Antibody
Clone ID: EPR4022
Swiss Prot: P27695
Mol Weight: 36kDa
Size: 100ul

Description
Apurinic endonuclease (Ape 1) functions as a major DNA repair enzyme that is essential for embryogenesis and cell viability (1). It is involved in the base excision repair (BER) pathway and acts as a reduction-oxidation signaling factor to maintain transcription factors in an active reduced state (2). Ape 1 plays a critical role as a transcriptional coactivator for different transcription factors (i.e. AP-1, NF-kappaB, p53) in controlling different cellular processes such as apoptosis, proliferation, and differentiation (3). Elevated levels of Ape 1 have been found in cancers such as ovarian, cervical, and prostate (4).

Recommended Applications
WB, IHC

Applications and Recommended Dilution Factors
WB: 1:10,000-50,000
IHC: 1 : 100 - 250

Species Reactivity
Human, Mouse, Rat

Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

Products Data  

 

Western blot analysis on (A) K562, (B) HepG2, and (C) Raji cell lysates using anti-Ape 1 RabMAb (cat. #2851-1).
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Ape 1 RabMAb (cat. #2851-1).
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Ape 1RabMAb (cat. #2851-1).

Specificity
A synthetic peptide corresponding to residues near the N-terminus in human Ape 1 was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
APEX1 antibody, APE antibody, APE1 antibody, APEN antibody, APEX antibody, APX antibody, HAP1 antibody, REF1 antibody, DNA-(apurinic or apyrimidinic site) lyase, mitochondrial antibody, REF-1 antibody, Redox factor-1 antibody

Description References
1. Manvilla et al. Biomol NMR Assign. 2009. 2. Fishel et al. DNA Repair (Amst). 7(2): 177-186, 2008. 3. Tell et al. Antioxid Redox Signal. 7(3-4):367-84, 2005. 4. Evans et al. Mutat Res. 461(2):83-108, 2000.