Apaf-1(APAF1)antibody
Cat.#: 1038-1
Rabbit Monoclonal Antibody
Clone ID: E38
Swiss Prot: O14727
Mol Weight: 130kDa
Size: 100ul

Description
Apaf-1, or apoptotic protease activating factor 1, is a human protein homolog of the C. elagans apoptotic regulatory protein CED-4 that activates caspase-9 during apoptosis (1). Cytosolic Apaf-1 forms an oligomeric complex with caspase-9 in the presence of cytochrome c and dATP, which leads to caspase-9 activation and subsequent activation of caspase-3 (2, 3). Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains, consequently enhancing the catalytic activity of caspase-9 (4), leading to activation of the caspase signaling cascade and apoptosis.

Recommended Applications
WB, IHC, ICC

Applications and Recommended Dilution Factors
WB: 1:500
IHC: 1:50-1:100
ICC: 1:50-1:100

Species Reactivity
Human, Mouse

Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

Products Data 

 

A. Western blot analysis on HeLa cell lysate using anti-Apaf-1 RabMAb (cat. #1038-1); dilution 1:500.
B. Immunohistochemical analysis of paraffin-embedded stomach adenocarcinoma using anti-Apaf-1 RabMAb (cat. #1038-1).

Specificity
A synthetic peptide corresponding to residues near N-terminus of human Apaf-1 was used as immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
APAF1 antibody, APAF-1 antibody, CED4 antibody, DKFZp781B1145 antibody, Apoptotic protease-activating factor 1 antibody, antibody

Description References
1. Zou, H. et al. Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 90(3): 405
2. Saleh, A. et al. Cytochrome c and dATP-mediated oligomerization of Apaf-1 is a prerequisite for procaspase-9 activation. J. Biol. Chem. 274 (25): 17941
3. Hu, Y. et al. Role of cytochrome c and dATP/ATP hydrolysis in Apaf-1-mediated caspase-9 activation and apoptosis. EMBO J. 18(13): 3586
4. Shiozaki, E.N. et al. Oligomerization and activation of caspase-9, induced by Apaf-1 CARD. PNAS 99(7): 4197

Product References
1. Kamitsuji Y, Kuroda J, Kimura S, et al, The Bcr-Abl kinase inhibitor INNO-406 induces autophagy and different modes of cell death execution in Bcr-Abl-positive leukemias ,Cell Death Differ 15 (2008) 1712-1722
[Application: WB]