Akt1 Phospho(pT308)(AKT1)antibody
Cat.#: 2214-1
Rabbit Monoclonal Antibody
Clone ID: EP2107Y
Swiss Prot: P31749
Mol Weight: 60kDa
Size: 100ul

Description
Akt1/PKBa is a Ser/Thr protein kinase, whose kinase activity plays a key role in various cellular functions, including apoptosis, glycogen synthesis, and cell growth. Akt1/PKBa is activated by various growth and survival factors (1). Phosphorylation on Thr308, Ser473 and Tyr474 is required for full activity. Akt1/PKBa inhibits apoptosis by phosphorylating and inactivating several targets, including caspase-9 (2). Akt1/PKBa regulates glycogen synthesis by phosphorylating and inactivating glycogen synthase kinase-3a and b (3, 4). Three mammalian Akt1/PKBa isoforms have been identified.

Recommended Applications
WB

Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000

Species Reactivity
Human, Mouse

Cross reactivity determined by western blot only.

Products Data

A. Western blot analysis on NIH 3T3 cell lysates using anti-Phospho-Akt1 (pT308) RabMAb (cat. #2214-1), 1:10,000 dilution. Cells were either (A) untreated (B) treated with PDGF

Specificity
A phospho specific peptide corresponding to residues surrounding Threonine 308 of human Akt1 was used as immunogen. The antibody detects Akt1 phosphorylated at Threonine 308.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
AKT1 antibody, AKT antibody, MGC99656 antibody, PKB antibody, PKB-ALPHA antibody, PRKBA antibody, RAC antibody, RAC-ALPHA antibody, RAC-alpha serine/threonine-protein kinase antibody, Proto-oncogene c-Akt antibody, RAC-PK-alpha antibody

Description References
1. Burgering, B.M. and P.J. Coffer.Nature 376: 599
2. Cardone, M.H., et al. Science 282: 1318
3. Hajduch, E., et al. FEBS Lett. 492: 199
4. Cross, D.A., et al. Nature 378: 785-789, 1995.

Product References
1. Feng Q, Di R, Tao F, et al, PDK1 Regulates Vascular Remodeling and Promotes Epithelial-Mesenchymal Transition in Cardiac Development ,Mol Cell Biol 30 (2010) 3711-3721