Adducin alpha Phospho(pS59)(ADD1)antibody
Cat.#: 2208-1
Rabbit Monoclonal Antibody
Clone ID: EP2175AY
Swiss Prot: P35611
Mol Weight: 115kDa
Size: 100ul

Description
Alpha adducin is a 80-120 kD member of the aldolase class II family and adducin subfamily. Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes (1-2). Adducin is a heterodimeric protein that consists of related subunits; α and β, or α and γ subunits (3). Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinase C (PKC), and protein kinase A (PKA) (4). PKA specifically phosphorylates Ser59, Ser408, Ser436, and Ser481 located in the neck domain of alpha Adducin. Phosphorylation of Adducin alpha by Rho-kinase at Thr445 and Thr480 enhances the F-actin-binding activity of Adducin alpha (5).

Recommended Applications
WB, IP

Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IP: 1:40

Species Reactivity
Human

Cross reactivity determined by western blot only.

Products Data

A. Western blot analysis on HeLa cell lysates using anti-Phospho-Adducin alpha (pS59) RabMAb (cat. #2208-1), 1:10,000 dilution. Cells were either (A) untreated (B) treated with calyculin A

Specificity
A synthetic phospho-peptide corresponding to residues surrounding Ser59 of human alpha Adducin was used as an immunogen. The antibody only detects alpha Adducin phosphorylated on Serine 59.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ADD1 antibody, ADDA antibody, MGC3339 antibody, MGC44427 antibody, Alpha-adducin antibody, Erythrocyte adducin subunit alpha antibody

Description References
1. Gardner K, Bennett V., J Biol Chem 261:1339-1348, 1986.
2. Kuhlman PE, et al. J Biol. Chem, 271: 7986-7991, 1996.
3. Dong, LC, et al. J Biol Chem 270: 25534-25540, 1995.
4. Palfrey HC, et al. J Biol Chem. 260:16021-16029, 1985.
5. Fukata, et al. J Cell Biol, 145:347-361, 1995.