ACHR-alpha 4(CHRNA4)antibody
Cat.#: 3528-1
Rabbit Monoclonal Antibody

Clone ID: EPR4563(2)
Swiss Prot: P43681
Mol Weight: 70kDa
Size: 100ul

Description
ACHR-alpha 4 is a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in ACHR-alpha 4 cause nocturnal frontal lobe epilepsy type 1. Polymorphisms in this protein that provide protection against nicotine addiction have been described (1).

Recommended Applications
WB, IP

Applications and Recommended Dilution Factors
WB: 1:10,000 - 50,000
IP: 1:10 - 100

Species Reactivity
Human, Mouse, Rat

Species cross-reactivity determined by western blot only

Products Data

Western blot analysis on (A) SH-SY5Y, (B) A673, (C) C6, and (D) Neuro-2a cell lysates using anti-ACHR-alpha 4 RabMAb (cat. #3528-1).

Specificity
A synthetic peptide corresponding to residues in human ACHR-alpha 4 was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
CHRNA4 antibody, BFNC antibody, EBN antibody, EBN1 antibody, FLJ95812 antibody, NACHR antibody, NACHRA4 antibody, NACRA4 antibody, Neuronal acetylcholine receptor subunit alpha-4 antibody, antibody

Description References
1. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1; 33: D54-8.