For the isolation and enrichment of FAT10 interacting proteins. FAT10 agarose conjugate may have potential utility for the capture/pull down of FAT10-interacting partners, including processing/signaling pathway proteins and FAT10 target proteins, from cell lysates and extracts by affinity binding. The immobilized protein retains the exposed C-terminal diglycine required for FAT10 modification and therefore may be suitable for in vitro FAT10 conjugation. It is anticipated that captured proteins may be eluted for identification, analysis or subsequent biochemical reaction.
Product Specification
Source/Host: Human recombinant FAT10 coupled to agarose at 0.5mg protein per ml settled resin.
Application: Capture and analysis of FAT10-interacting proteins from cell/tissue lysates. Isolation of specific FAT10-interacting proteins for use in subsequent biochemical experiments.Identification of novel FAT10-interacting partners.Identification of potential FAT10 substrates through covalent addition to FAT10 target proteins.
Long Term Storage: +4°C
Miscellaneous/General: FAT10 agarose conjugate may have potential utility for the capture/pull down of FAT10-interacting partners, including processing/signaling pathway proteins and FAT10 target proteins, from cell lysates and extracts by affinity binding. The immobilized protein retains the exposed C-terminal diglycine required for FAT10 modification and therefore may be suitable for in vitro FAT10 conjugation. It is anticipated that captured proteins may be eluted for identification, analysis or subsequent biochemical reaction.