BML-PW9780-0100 100 µl
BML-PW9780-0025 25 µl
Ub+1 is a novel form of ubiquitin that has been selectively observed in the brains of Alzheimer’s patients, including those with nonfamilial Alzheimer’s disease. Ub+1 is an efficient substrate for polyubiquitinylation in vitro and in transfected human cells. The resulting polyubiquitin chains are refractory to disassembly by deubiquitinating enzymes and potently inhibit the degradation of a polyubiquitinylated substrate by purified 26S proteasomes. Thus, expression of Ub+1 in aging brain could result in dominant inhibition of the Ub-proteasome system, leading to neuropathologic consequences.
Expression of Ub+1 occurs through a phenomenon known as molecular misreading, in which non-DNA-encoded dinucleotide deletion(s) are found in mRNA within or adjacent to GAGAG motifs. A mechanism by which Ub+1 expression could lead to dominant inhibition of the Ub-proteasome system has been demonstrated.
Product Specification
Alternative Name: Ubiquitin+1
Formulation: Liquid. In PBS containing 10mM sodium azide.
Immunogen: Synthetic peptide corresponding to aa 76-95 of human Ub+1.
Source/Host: From rabbit.
Specificity: Recognizes human Ub+1.
Application: Immunohistochemistry
Western Blot (1:1000)
Optimal conditions must be determined individually for each application.
Shipping: SHIPPED ON BLUE ICE
Long Term Storage: -20°C
Use/Stability: Store unopened vial at -20°C until required for use.  The use of high quality ‘antiserum-grade’ plastic or glass vials is recommended.. Dilute to working strength with phosphate buffered saline pH 7.2-7.4 and 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used). Store diluted antibody at 2-4°C (do not freeze) and use within 1 month.
Handling: After opening, prepare aliquots and store at -20°C.
Do not freeze. 
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