活力定义:25°C时,pH7.65,每分钟催化1 μmol 马尿酰-L-精氨酸水解的酶量定义为一个酶活力单位
来源:猪胰。
介绍:Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and ornithine from the C-terminal end of polypeptides. The molecular weight is 34,500 daltons, the pH optimum is 7.9, and the pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine, lysine and ornithine. The enzyme is not inhibited by di-isopropylfluorophosphate (DFP), but it is inhibited by metal chelating agents, e.g., 1,10-phenanthroline.
储存条件:-20℃
外观:液体
用途:可专一性地从肽链的C端开始逐个降解,释放出游离氨基酸的一类肽链外切酶。羧肽酶B只水解以碱性氨基酸(如精氨酸和赖氨酸)为C末端残基的肽键。