存贮条件 | 储存温度 -20°C |
产品介绍 | Lactoperoxidase (LPO) is a glycoprotein with a hemin prosthetic group which may occur as a mixture of two isozymes. It has a molecular weight of 77,500 daltons. LPO catalyzes the hydrogen peroxide oxidation of iodide according to the following reaction: 2I- + H2O2 + 2H+ → I2 + 2H2O. Iodide reacts directly with the heme group; upon addition of H2O2 the complex iodinates the substrate. LPO is inhibited by hydrazines. The assay procedure has been updated from that of Morrison to an ABTS?/H2O2 based method with increased sensitivity and reproducibility. |
备注 | Chromatographically purified. A lyophilized powder. |
生化机理 | 乳过氧化物酶催化过氧化氢与碘化物的氧化反应生成碘。该活性提供了一种温和的特异性氯胺 T 替代物,用于蛋白质和 DNA 的放射性碘化反应。 |
别名 | 供体:过氧化氢氧化还原酶;过氧化物酶;HRPLPO;Peroxidase;Donor:hydrogen peroxide oxidoreductase |