产品描述
| 敏感性 | 易吸潮 |
| 存贮条件 | 储存温度 -20°C |
| 应用 | Catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing ATP as a phosphate source. Used to determine glucose, fructose, mannose and ATP. |
| 产品介绍 | Hexokinase catalyzes the reaction:D-hexose + ATP → D-hexose 6-phosphate + ADP Hexokinases have been isolated from the yeast cell in two distinct forms, designated P-I and P-II (Schulze et al. 1969). These are separate, noninterconvertible isozymes (Womack et al. 1973). Hexokinase is used to determine glucose, fructose, mannose and ATP. |
| 备注 | Chromatographically purified. A lyophilized powder containing ~30% buffer/stabilizer. Phosphohexose isomerase, 6-phosphogluconate dehydrogenase, adenylate kinase and creatine phosphokinase contaminant activities are 0.10%, 0.005%, 0.005% and 0.005%, respectively. |
| 生化机理 |
Catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing ATP as a phosphate source. The rate of phosphorylation varies with different hexoses (pH 7.5, 30 °C):D-fructose KM: 0.33 mM;D-glucose KM: 0.12 mM;D-mannose KM: 0.05 mM. Yeast hexokinase exists as two similar isoforms, PI and PII (A and B), with isoelectric points of 5.25 and 4, respectively.Molecular Weight: ~ 54 kDa (monomer);~110 kDa (dimer);Optimal pH: 7.5 to 9.0;Extinction Coefficient: E1% = 8.85 (PI) and 9.47 (PII) at 280 nm. Activators: Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds. Inhibitors: sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose?, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate). |
| 别名 | 己糖激酶;ATP:D-Hexose-6-phosphotransferase;Hexokinase from Saccharomyces cerevisiae;Hexokinase |
