脱氧核糖核酸酶Ⅱ 来源于猪脾(纯化,溶液)

脱氧核糖核酸酶Ⅱ 来源于猪脾(纯化,溶液)

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D128605-5KU 咨询
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属性:产地:上海; 品牌:阿拉丁 ;货期:现货

上海阿拉丁生化科技股份有限公司

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存贮条件 储存温度 -20°C

描述

应用 DNase II has been used to treat transformed cells during the purification of β-lactamase. It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA).
Deoxyribonuclease II from bovine spleen has been used in a study that conducted a partial purification of deoxyribonucleases from eggs and liver of Xenopus laevis. Deoxyribonuclease II from bovine spleen has also been used in a study to investigate nucleic acid and protein synthesis of splenic lymphocytes.
产品介绍 Deoxyribonuclease II from porcine spleen has a molecular weight of 38 kDa. The enzyme is a glycoprotein endonuclease with dimeric structure. Optimum pH range is 4.5-5.0 at ionic strength 0.15 M. Deoxyribonuclease II (Acid DNase) hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3'-phosphates. It also acts on p-nitrophenylphosphodiesters at pH 5.6-5.9. Bernardi (BBRC, 17, 573, 1971) describes a three stage degradation of native DNA by DNase II.
备注 Chromatographically prepared. A solution in 50% glycerol.
生化机理 The enzyme is a lysosomal acid hydrolase that hydrolyzes DNA to 3′-phosphoryl oligonucleotides in the absence of metal ions, under acidic conditions. It is a non-covalently linked α·β heterodimer of approximately 45,000 Da molecular mass.
别名 ;DNase II;Deoxyribonucleate 3′-oligonucleotido-hydrolase
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