产品描述
| 存贮条件 | 储存温度 -20°C |
| 应用 | 用于以小型化的高通量筛选形式测定羧肽酶B活性,评估C-末端赖氨酸在人纤溶酶原结合至双歧杆菌过程中的影响。使用流式细胞术对用羧肽酶B（一种C-末端赖氨酸特异性内肽酶）处理的效果进行分析和检测。羧肽酶B已被用于开发用于圈养和野生北极熊的非侵入性妊娠试验。羧肽酶B已被用于确定急性胰腺炎的新潜在生物标志物。 |
| 产品介绍 | Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and ornithine from the C-terminal end of polypeptides. The molecular weight is 34,500 daltons, the pH optimum is 7.9, and the pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine, lysine and ornithine. The enzyme is not inhibited by di-isopropylfluorophosphate (DFP), but it is inhibited by metal chelating agents, e.g., 1,10-phenanthroline. |
| 备注 | Chromatographically purified. A solution in 100mM sodium chloride. Chymotrypsin and trypsin 0.02%. |
| 生化机理 | 羧肽酶B是一种蛋白水解酶，能够快速水解肽键，从而从肽和蛋白质中释放某些羧基末端碱性氨基酸。其分子量为34,300±600 Da。其每摩尔含有一克不可透析的锌原子。金属螯合剂1,10-菲咯啉、8-羟基喹啉-5-磺酸、和2,2′-联吡啶可抑制该酶活性。 |
| 别名 | 肽基-L-赖氨酸（L-精氨酸）水解酶;Peptidyl-L-lysine(L-arginine) hydrolase;Protaminase |
