Poster

Integrating shotgun lipidomics and HPLC separation of lipids into a new platform for lipid analysis　　

【Summary 】

• Development of a lipidomics platform based on direct infusion and LC MS analysis
• LC MS analysis by normal phase chromatography and nanoflow split to a chip-based infusion device 
• Implementation of a fraction collection strategy for indepth profiling of lipid species by direct infusion analysis 
• This platform is a powerful tool for arraying the lipidome of poorly characterised organisms 

Ion source/Fraction collector: NanoMate Triversa Sample analysis was performed by LC-coupling/fraction collection mode using a post-column split where 50 nl/min was directed to the ESI-chip and 49.5 µl/min was use for parallel fraction collection.