Application Note   Lipidomics Evaluating Liquid Extraction Surface Analysis (LESA) for Comparative Lipidomics Profiling   Introduction   Professor  Manuel  Mayr  and  his  group  at the  King’s  British  Heart  Foundation  Center of  King’s  College  London  evaluated  liquid  extraction  surface  analysis  (LESA)  compared to   previously   published   tissue   extraction techniques.  The  TriVersa  NanoMate  LESA  capability was employed to provide automated and efficient sampling. In addition, it allowed for easy analysis of one sample to act as its  own control.  The group was able to evaluate the ionization efficiency in both negative and positive mode, thereby increasing the amount  of information obtained.   By comparing LESA to previously published tissue extraction technique of shotgun lipidomics, the group was able to show comparable and often times better data without sample preparation, thereby saving time and money.   质谱：TSQ Vantage三重四极杆，nanomate   结论：  

• Liquid extraction surface analysis (LESA) is a rapid approach to tissue analysis without sample preparation time and effort. 
• Shotgun  lipidomics  provides  valuable  insights  into  the  relationship  within  artherosclerotic plaques.
• LESA and other advanced mass spectrometry techniques can be employed for diagnostic and drug screening applications due to their complete profiling of lipid species and classes.