Anal Chem 2007 Jun;79 (12): 4595-602. Dynamic range and mass accuracy of wide-scan direct infusion nanoelectrospray fourier transform ion cyclotron resonance mass spectrometry-based metabolomics increased by the spectral stitching method. Southam AD , Payne TG , Cooper HJ , Arvanitis TN , Viant MR .

School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.

Abstract Direct infusion nanoelectrospray Fourier transform ion cyclotron resonance mass spectrometry (DI nESI FT-ICR MS) offers high mass accuracy and resolution for analyzing complex metabolite mixtures. High dynamic range across a wide mass range, however, can only be achieved at the expense of mass accuracy, since the large numbers of ions entering the ICR detector induce adverse space-charge effects. Here we report an optimized strategy for wide-scan DI nESI FT-ICR MS that increases dynamic range but maintains high mass accuracy. It comprises the collection of multiple adjacent selected ion monitoring (SIM) windows that are stitched together using novel algorithms.

FT-ICR Mass Spectrometry. Samples were analyzed using a  hybrid  7-T  Fourier  transform  ion  cyclotron  resonance  mass spectrometer (LTQ FT, Thermo Electron Corp., Bremen, Ger-many) equipped with a chip-based direct infusion nanoelectrospray ionization assembly (NanoMate, Advion Biosciences, Ithaca, NY).