TNF信号通路PCR芯片可以同时测定84个与TNF信号通路相关的关键基因。这些关键基因参与了TNF配体、受体信号通路，或者是表达受到了TNF信号通路的调控。其中包括TNF超家族、TNF受体超家族、以及与TNFR1和TNFR2信号通路相关的基因。通过实时定量PCR的方法，研究者即能够利用该芯片简单可靠地同时检测TNF信号通路相关基因的表达。
TNF Superfamily Members: Induction of Apoptosis: FASLG, LTA, TNFSF10, TNFSF14, TNFSF8. Caspase Activation: TNFSF15. Caspase Inhibition: TNFSF14. Anti-apoptosis Genes: CD40LG, TNF, TNFSF18. Other Apoptosis-Related Genes: CD70 (TNFSF7), TNFSF9. Inflammatory Response: CD40LG, TNF. NFκB Signaling Pathway: FASLG, TNF, TNFSF10, TNFSF14, TNFSF15. Other TNF Superfamily Members: LTB,PGLYRP1, TNFSF11, TNFSF12, TNFSF13, TNFSF13B, TNFSF4, PSMG2. TNF Receptor Superfamily Members: Induction of Apoptosis: FAS, TNFRSF10A, TNFRSF10A, TNFRSF10B, TNFRSF19, TNFRSF25, CD27 (TNFRSF7), TNFRSF9, TRADD. Caspase Activation: TNFRSF10A, TNFRSF10B. Caspase Inhibition: CD27 (TNFRSF7). Anti-apoptosis Genes: FAS, TNFRSF10D, TNFRSF18, TNFRSF6B, CD27 (TNFRSF7). Other Apoptosis Genes: CD40, LTBR, NGFR, TNFRSF10C, TNFRSF11B, TNFRSF12A, TNFRSF14, TNFRSF1A, TNFRSF1B, TNFRSF21. Inflammatory Response: CD40, TNFRSF1A. NFκB Signaling Pathway: CD40, EDA2R, LTBR, TNFRSF10A, TNFRSF10B, TNFRSF1A, CD27 (TNFRSF7), TRADD. JNK Signaling Pathway: EDA2R, TNFRSF19, CD27 (TNFRSF7). Other TNF Receptor Superfamily Members: TNFRSF11A, TNFRSF13B, TNFRSF13C, TNFRSF17, RELT, TNFRSF4, TNFRSF8. TNFR1 Signaling Pathway: Induction of Apoptosis: CASP3, CRADD, FADD, TRADD. Caspases: CASP2, CASP3, CASP8. Anti-apoptosis Genes: BAG4, CASP2, TNF. Other Apoptosis Genes: DFFA, PAK1, TNFRSF1A, TRAF2. Inflammatory Response: TNF, TNFRSF1A. NFκB Signaling Pathway: CASP8, FADD, TNF, TNFRSF1A, TRADD. JNK Signaling Pathway: MAP2K4, MAPK8, PAK1. Transcription Regulators: JUN, PARP1, RB1, TNF, TNFRSF1A. TNFR1 Signaling Pathway: ARHGDIB, CAD, HRB, LMNA, LMNB1, LMNB2, MADD, MAP3K1, MAP3K7, PAK2, PRKDC, SPTAN1. TNFR2 Signaling Pathway: Induction of Apoptosis: IKBKG, LTA, TRAF3. Anti-apoptosis Genes: NFKB1, TNFAIP3. Other Apoptosis Genes: NFKBIA, TNFRSF1B, TRAF1, TRAF2. Inflammatory Response: NFKB1. NFκB Signaling Pathway: CHUK, IKBKB, IKBKG, NFKBIA, TNFAIP3. Transcription Regulators: IKBKB, IKBKG, NFKB1, NFKBIA. TNFR2 Signaling Pathway: DUSP1, AGFG1, IKBKAP, MAP3K1.