克隆氏症PC芯片可用于研究与克隆氏症相关的84个基因的表达情况。
 The Human Crohn's Disease RT² Profiler PCR Array profiles the expression of 84 key genes differentially expressed during inflammatory bowel disease (IBD). The most common form of IBD is Crohn's disease, an inflammatory disorder of the intestines. Ulcerative colitis (UC), another common form of IBD, only affects the mucosa of the large intestine. The major symptoms of IBD include abdominal pain, vomiting, and diarrhea. The disease can result in weight loss, and during severe cases the patient may undergo complications such as decreased intestinal function and ultimately colectomy. Although the initial cause of IBD is not generally understood, the resulting biology is under investigation to research potential therapies as well as biomarkers for the various types of IBD. One major therapeutic goal is to reduce IBD symptoms and inflammation, since the symptomatic diseases have an increased risk of colorectal cancer. Inflammatory cytokines are upregulated, as are extracellular remodeling enzymes. The immune response is also upregulated, including innate immunity and T cell responses. Microarray studies have compared both Crohn's disease and UC to controls and each other to attempt to identify the underlying causes. This array includes genes identified via microarray to be differentially regulated during Crohn's disease and UC. A set of controls present on each array enables data analysis using the ΔΔCT method of relative quantification, assessment of reverse transcription performance, genomic DNA contamination, and PCR performance. Using real-time PCR, research studies can easily and reliably analyze the expression of a focused panel of genes involved in inflammatory bowel disease with thisarray.
Apoptosis: CASP1 (ICE), CCL2 (MCP-1), EGR3, IL1B, IL2RA (CD25), IL6, ITGB2, LCN2 (NGAL), SOD2, TNF.
Inflammation: C3, CCL11 (Eotaxin), CCL2 (MCP-1), CCL20 (MIP-3A), CCL25 (TECK), CCL5 (RANTES), CCR1, CCR2, CCR5, CX3CL1, CXCL1, CXCL10 (INP10), CXCL11 (I-TAC/IP9), CXCL2, CXCL3, CXCL9 (MIG), CXCR1 (IL8RA), FPR1, IL13, IL17A, IL1B, IL2RA (CD25), IL23A, IL5, IL6, IL8, ITGB2, LTB, LYZ, S100A8, S100A9, SAA1, SELE, STAT3, TNF.
Immunity:
Humoral: CCL2 (MCP-1), IL6, DEFA5, DEFA6, LYZ.
Innate: C3, C4BPB, CASP1, CD55, CR2, IL1RN, IRF5, ISG15 (G1P2), LCN2 (NGAL), NOD2, NOS2 (iNOS), SAA1, TYK2.
Adaptive: CCL11 (Eotaxin), CCR2, CCR5, CCR9, CXCR3, HLA-DQA1, HLA-DRA, HLA-DRB1, IFNG, IL13, IL17A, IL2RA (CD25), IL5, IL6, IRF5, STAT1.
Adhesion & Remodeling: CCL2 (MCP-1), CCR1, CCL11 (Eotaxin), CSTA, CX3CR1, CXCL12 (SDF1), CXCR3, ITGB2, MMP1, MMP10, MMP3, MMP7, PECAM1, SELE, SELL (LECAM1), TIMP1, VWF.
Unfolded Protein Response: HSP90B1 (TRA1), HSPA5 (GRP78), UBD.
Digestive Secreted Proteins: GCG, MUC1, REG1A, REG1B, TFF1 (pS2).
Metabolism: ALDOB, PCK1, TDO2.
Other Genes: ABCB1 (MDR1), ATG16L1, CHI3L1, EDN3, NR3C2.