Human Polycomb & Trithorax Complexes ChIP PCR Array

 ProductSpeciesTechnologyCat. No.Polycomb & Trithorax Complexes EpiTect ChIP qPCR ArrayHumanHistone ModificationsGH-506APolycomb & Trithorax Complexes EpiTect ChIP qPCR ArrayMouseHistone ModificationsGM-506A The Human Polycomb & Trithorax Complexes EpiTect Chip qPCR Array profiles the histone modification status or “histone code” of 84 genes encoding key polycomb and trithorax complex components including chromatin modification enzymes and remodeling factors. Histone modifications define chromatin structure, regulate gene activity and specify cellular status. The polycomb and trithorax complexes maintain epigenetic control of cell type specific gene expression patterns important for cellular identity via histone modification. The polycomb complex causes transcriptional repression, while the trithorax complex reverses that effect to maintain an active state of transcription. Dysregulation of these activities promotes oncogenesis by causing inappropriate expression of cell identity, differentiation and proliferation genes. Cancer research studies describe mutations in genes encoding polycomb and trithorax complex components, as well as complex regulators, during oncogenic transformation. Because these complexes play a central role in the epigenetic regulation of oncogenes and tumor suppressor genes, epigenetics may also stabilize their expression patterns, making an analysis of their own "histone code" also essential. This array includes core components of the polycomb and trithorax complexes as well as key interactors and genes necessary for complex assembly. Using chromatin immunoprecipitation and this real-time PCR Array, you can easily and reliably analyze the histone modification patterns associated with a focused gene panel involved in Polycomb and Trithorax complex activity.    

Polycomb Complexes: 
Core Components: BMI1 (PCGF4), EED, EZH2, PHC1, PHC2, PHC3, RBBP4, RBBP7, RING1, RNASEL, SCMH1. 
Alternate Core Components: CBX5, EZH1, PHF1, RNF2. 
Additional Complex Components: CBX8, DNMT1, DNMT3A, DNMT3B, E2F6, KDM2B, KDM5D, PCGF1, PCGF2 (RNF110), PHC2, RING1, RNF2, RNF2, RYBP. 
Polycomb Cofactors: 
Complex Assembly: AEBP2, RBP2, SNAI1, ZBTB16. 
Complex Interacting Genes: ARID1B, ASXL1, ASXL2, ASXL3, BAP1, CBX1, CBX2, CBX3, CBX4, CBX7, CTBP2, DNMT3L, EPC1, EPC2, HTT, INO80 (INOC1), INO80B, INO80C, INO80D, JARID2, L3MBTL2, LARP7, MBTD1, MLL4, MLL5, MOV10, MTF2, PCGF5, PHF19, PPP1CC, PPP1R8, SCML2, SIRT1, SMARCA4, SMARCC1, TRIM27, USP11, USP7, YAF2, YY1AP1, YY2. 
Trithorax Complexes: 
Core Components: ARID1A, ASH2L, CXXC1, HLTF, MLL2, MLL3, PBRM1, RBBP4, RBBP5, RBBP7, SMARCA1, SMARCA2, SMARCB1, SMARCC2, WDR5. 
Complex Interacting Genes: ARID1B, MLL4, SMARCA4, SMARCA5, SMARCC1.