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TGF / BMP Signaling Pathway PCR Array
TGF/ BMP信号通路PCR 芯片
TGFβ/BMP Signaling Pathway Plus RT² Profiler PCR Array profiles the expression of 84 genes related to TGFβ/BMP-mediated signal transduction. It also determines whether TGF pathway activity is activated, repressed, or unchanged in experimental samples. The array includes members of the TGFβ superfamily of cytokines and their receptors. SMAD and SMAD target genes are included. Related genes including adhesion and extracellular molecules and transcription factors are included. Some of the genes involved in downstream cellular and developmental processes are also represented. The array also includes 16 experimentally derived Signature Biomarker Genes which, along with classification algorithms, are used to generate the activity score. A set of controls present on each array enables data analysis using the ΔΔCT method of relative quantification, assessment of reverse transcription performance, genomic DNA contamination, and PCR performance. Using real-time PCR, research studies can easily and reliably determine TGF signaling pathway activity and analyze expression of a focused panel of genes related to TGFβ/BMP mediated signal transduction with this array.
TGF/BMP信号通路PCR芯片PCR芯片可用于研究TGF./BMP介导的信号转导相关的84个基因的表达，同时测定实验组样本中TGF信号通路活性是被激活，抑制还是没有变化。该芯片涵盖了TGF.超级家族的细胞因子和受体。SMAD和SMAD靶标基因，关联基因包括粘连因子、胞外信号分子以及转录因子都包含其中。一些下游细胞活动和发育过程相关的基因也包含其中。该芯片同时包含了16个通过实验验证得到的显著标志基因，通过分级运算程序可计算得到活性分值。每块芯片上都设定了一组对照通过ΔΔCT方法分别对反转效率，基因组DNA含量和PCR效率进行评估。通过实时定量PCR，使用者通过实时定量PCR，使用者即能够利用该芯片简单可靠地测定TGF信号通路活性并同时检测
TGF./BMP介导的信号转导相关基因的表达。
TGFß Superfamily Ligands:
Activin:INHA, INHBA, INHBB.
BMP:AMH, BMP1, BMP2, BMP3, BMP4, BMP5, BMP6, BMP7, GDF2 (BMP9), GDF3 (VGR-2), GDF5 (CDMP-1), GDF6, GDF7.
Nodal:NODAL.
TGFβ:TGFB1, TGFB2, TGFB3.
Inhibitor/Cofactors:BAMBI, CHRD, DCN, FST, LEFTY1, LTBP1, LTBP2, NOG, TGFBI, TGFBRAP1, THBS1, TGIF1.
TGFß Superfamily Receptors:
Activin:ACVR1 (ALK2), ACVR2A, ACVRL1 (ALK1).
BMP:AMHR2, BMPR1A (ALK3), BMPR1B (ALK6), BMPR2.
TGFβ:TGFBR1, TGFBR2, TGFBR3.
Transcription Factors and Regulators:DLX2, FOS, GSC, HIPK2, ID1, JUN, JUNB, MYC, RUNX1 (AML1), SMAD1 (MADH1), SMAD2 (MADH2), SMAD3 (MADH3), SMAD4 (MADH4), SMAD5 (MADH5), SMAD7, SOX4, STAT1, TGFB1I1, TSC22D1 (TGFB1I4).
SMAD Target Genes:
TGFβ/Activin-Responsive:ATF4, CDKN1A (p21WAF1/p21CIP1), CDKN1B (p27KIP1), CDKN2B (p15INK2B), COL1A1, COL1A2, DCN, EMP1, FOS, GADD45B, GSC, HERPUD1, IFRD1, IGF1, IGFBP3, IL6, JUN, JUNB, MYC, PDGFB, SERPINE1 (PAI-1), TGFB1I1, TNFSF10, TSC22D1 (TGFB1I4), TGFBI, TGIF1.
BMP-Responsive:BGLAP (Osteocalcin), DLX2, ID1, ID2, JUNB, SOX4, STAT1.
Molecules Regulating Signaling of the TGF-ß Superfamily:BAMBI, BMPER, CDKN2B (p15INK2B), CHRD, ENG (Evi-1), MECOM, FST (Follistatin), HIPK2, NOG, PLAU (uPA), RUNX1 (AML1), SMURF1.
Adhesion and Extracellular Molecules:
Adhesion Molecules:BGLAP (Osteocalcin), ENG (Evi-1), TGFB1I1, TGFBI.
Extracellular Matrix Structural Constituents:BGLAP (Osteocalcin), COL1A1, COL1A2, LTBP1, LTBP2, TGFBI.
Genes Involving in Cellular and Developmental Processes:
Apoptosis:CDKN1A (p21WAF1/p21CIP1), CDKN1B (p27KIP1), HIPK2, IGFBP3, INHA, INHBA, STAT1, TGFB1.
Embryonic Development:BMP4, INHBA, MECOM, SMAD3, SMURF1.
Muscle Development:DCN, IGF1, SMAD3.
Neurogenesis:DLX2, INHA, INHBA, NOG.
Reproduction:AMH, AMHR2, FST (Follistatin), INHA, INHBA, INHBB.
Skeletal Development:BGLAP (Osteocalcin), BMP1, BMP2, BMP3, BMP4, BMP5, BMP6, BMP7, BMPR2, CHRD, COL1A1, COL1A2, IGF1, INHA, INHBA, NOG.
Pathway Activity Signature Genes: ACTC1, BMPR2, EGR2, FAS, FGF2, FSTL3, JUNB, KANK4, KLHL24, NOV, PMEPA1, SERPINE1, SMAD6, SOX4, STK38L, UBASH3B.
