Human T Helper Cell Differentiation, Signature Panel: EAHS-341Z The Human T Helper Cell Differentiation EpiTect Methyl II Signature PCR Array profiles the promoter methylation status of a panel of 22 genes regulating the commitment of precursor T cells to differentiate into specific effector subtypes. The 2 most well-known and characterized T helper cell subtypes, Th1 and Th2 cells, mediate cellular and humoral immune responses, respectively. Many published research studies find that one subtype population and its immune response tend to dominate in immune disorders such as allergy, asthma, autoimmunity, diabetes, hypersensitivity, and rheumatoid arthritis. This array profiles key regulatory genes encoding mostly transcription factors and cytokines demonstrating differential methylation status or histone modifications during proliferating T cell differentiation into effector Th1 or Th2 cells. Profiling research samples containing genomic DNA from T cell population sources will help to correlate these epigenetic markers with experimentally-induced, subtype-specific immune responses. The results can also provide insights into the epigenetic regulation of the molecular mechanisms and biological pathways behind T helper cell lineage commitment decisions in mammalian model systems. With a simple restriction enzyme digestion and real-time PCR, research studies can analyze the promoter methylation status of 22 different genes involved in T helper cell differentiation with this DNA methylation PCR array.
Both 96-well and 384-well ( 4 X 96 ) formats are available    

Th1 Cells: EOMES, TBX21.
Th2 Cells: GATA3, IL13, PPARG.
Th17 Cells: RORA.
Inducible & Natural Regulatory T (iTreg & nTreg) Cells: FOSL1, IRF4, IRF8, MYB, NR4A3, POU2F2, REL, RELB, TGIF1, TNFSF11.
Conventional Versus Regulatory T Cells: CHD7, GATA4, HOXA10, ID2, LRRC32, PERP