Cellular Senescence PCR Array细胞衰老PCR芯片

 

Product

Species

Technology

Cat. No.

Cellular Senescence PCR Array

Human

Gene Expression

PAHS-050Z

Cellular Senescence PCR Array

Mouse

Gene Expression

PAMM-050Z

Cellular Senescence PCR Array

Rat

Gene Expression

PARN-050Z

细胞衰老PCR芯片可用于研究细胞衰老生物过程的84个关键基因的表达。衰老细胞通常呈现大而扁平的细胞外观，与其他细胞接触少，且细胞外基质粘附性增加。在分子层面，细胞衰老过程激活p53和pRb信号通路，zei终脱离正常细胞周期反应。正常情况下，细胞经历一定次数的复制后进入衰老；除此之外，压力诱导也会导致细胞提前衰老。这些压力包括DNA损伤、氧化应激、干扰素相关反应、IGF或MAPK信号。有人推测，衰老过程zei初是一种抗病毒机制。由于癌症早期会激活细胞衰老，癌症晚期细胞衰老会失调，因此理解和控制细胞衰老过程对癌症治疗有很大意义。这张芯片包含了参与原发性衰老和已报道的会导致细胞提前衰老的压力基因。通过实时定量PCR的方法，研究者即能够利用该芯片简单可靠地同时检测细胞衰老相关基因的表达。

Senescence Pathway: ATM, BMI1, CCND1, CCNE1, CDK2, CDK4, CDK6, CDKN1A, CDKN2A (p16INK4), CDKN2D, CHEK1, CHEK2, E2F1, E2F3, ETS1, ETS2, MDM2, RB1, RBL2, TP53, TWIST1.

Senescence Initiators:
p53/pRb Signaling (Cell cycle): ABL1, AKT1, ALDH1A3, CCNA2, CCNB1, CDC25C, CDKN1C, CDKN2B (p15INK4b), CDKN2C, CITED2, CREG1, GSK3B, ID1, IGF1R, IGFBP3, ING1, MAP2K6, MAPK14, MORC3, MYC, PCNA, PIK3CA, PLAU, RBL1, SERPINB2 (PAI-2), SERPINE1 (PAI-1), SIRT1, SPARC, TGFB1.
Interferon Related: ALDH1A3, CDKN1A, CDKN1B (p27KIP1), EGR1, IFNG, IGFBP3, IGFBP7, IRF3, IRF5, IRF7, NFKB1, RB1, SERPINB2 (PAI-2).
*** Growth Factor (IGF) Related: IGF1, IGF1R, IGFBP3, IGFBP5, IGFBP7.
Mitogen-Activated Protein Kinase (MAPK) Signaling: HRAS, MAP2K1, MAP2K3, MAP2K6, MAPK14.
Oxidative Stress: ALDH1A3, HRAS, MAPK14, NOX4, PRKCD, SOD1, SOD2.
DNA Damage: ATM, GADD45A, NBN, PCNA, TERF2, TERT, TP53BP1.

Senescence Responses:
p53 Effectors: ALDH1A3, E2F1, HRAS, IGFBP3, MYC.
p21 Effector: CALR.
p16 Effectors: TBX2, TBX3.
Cytoskeleton Related: AKT1, FN1, HRAS, PIK3CA, PLAU, SERPINE1 (PAI-1), SPARC, THBS1, VIM.
Cell Adhesion: CD44, COL1A1, COL3A1, TGFB1, TGFB1I1, THBS1.
Other: GLB1, PTEN.