黄斑退化PCR芯片可用于研究与老年性黃斑退化病理过程相关的84个基因的表达情况。
 

ProductSpeciesTechnologyCat. No.Macular Degeneration PCR ArrayHumanGene ExpressionPAHS-171ZMacular Degeneration PCR ArrayMouseGene ExpressionPAMM-171ZMacular Degeneration PCR ArrayRatGene ExpressionPARN-171Z

The Human Macular Degeneration RT² Profiler PCR Array profiles the expression of 84 genes involved in the pathogenesis of age-related macular degeneration (AMD). AMD usually affects older adults and can make it difficult or impossible to read or recognize faces, although enough peripheral vision remains to allow other daily life activities. AMD is an ocular disease that involves an aspect-specific region of the retina called the macula. The macula facilitates central vision and permits high-resolution visual acuity due to its dense concentration of cone photoreceptors. AMD starts with characteristic yellow deposits (drusen) in the macula between the retinal pigment epithelium and the underlying choroid, with pigmentary abnormalities. The late stage is divided into two groups: dry (non-exudative) and wet (exudative/neovascular) forms. The dry form is characterized by atrophic changes in the macula and clinically has a slower deterioration and better preservation of visual acuity. Wet AMD involves choroidal neovascularization, which is the formation of new abnormal blood vessels in the choriocapillaries through Brusch's membrane. These vessels have a greater tendency of leakage and bleeding into the macula, ultimately leading to irreversible damage to the photoreceptors if left untreated. The molecular pathways underlying AMD's onset and progression remain poorly delineated. The genes profiled with this array include inflammatory and endothelial cell markers for vascularization as well as AMD-associated markers for drusen, Brusch's membrane, and retinal abnormalities. A set of controls present on each array enables data analysis using the ΔΔCT method of relative quantification and assessment of reverse transcription performance, genomic DNA contamination, and PCR performance. Using real-time PCR, research studies can easily and reliably analyze the expression of a focused panel of genes involved inage-related macular degeneration with this array.
Anti-angiogenesis: SERPINE1 (PAI-1), SERPINF1, SERPING1.
Brusch's membrane markers: FN1, MMP2, MMP9, TIMP1, TIMP3.
Choroidal Neovascularization: IGF1, TGFB1, VEGFA.
Drusen Markers: APOE, C2, C3, C5, C9, CFB (BF), CFH, CFHR3, CFI, HMOX1, LIPC, TF, VTN.
Endothelial Function: ANXA5, CLU, COL14A1, CTGF, EFEMP1, ELN, FBLN5, ICAM1, NOS3 (eNOS), VCAM1, VIM, VWF.
Inflammatory Markers: CCL2 (MCP-1), IL6, IL8, PLG, TLR3, TLR4.
Oxidative Stress: DICER1, GSTM1 (MGST1), GSTP1, HIF1A, HMOX2, NOS1 (nNOS), SOD2.
Retinal Pigment Epithelium Markers: CCL11 (Eotaxin), CRP, CRYAA, CRYAB, CRYGD, CX3CR1, CXCL12 (SDF1), FASLG (TNFSF6), RHO, RLBP1, RPE65, SAG.
Other AMD-related Genes: ABCA1, ABCA4, ACE, ALB, ARMS2, CETP, CP, CST3, CTSD, ERCC6, FANCG, GFAP, HMCN1, HTRA1, LEP, LPL, PON1, SCARB1, SLC4A1, SPARC, STMN1, THY1, VLDLR.