前言：

　　High performance liquid chromatography – tandem mass spectrometry (HPLC-MS/MS) is now widely accepted for measurement of vitamin D metabolites. Many clinical research laboratories use 1-dimensional (1D) chromatography (for example, a single HPLC pump and chromatography column) with a triple stage quadrupole mass spectrometer. Various sample cleanup protocols, such as solid phase extraction (SPE), liquid-liquid extraction (LLE), and protein precipitation (PPT), have been applied in these analyses. Frequently, interfering peaks are seen in 25-OH vitamin D3 chromatograms, adversely affecting peak integration and leading to poor accuracy and reproducibility. Here we investigate the use of 2-dimensional chromatography using TurboFlow technology to remove all interfering peaks and significantly improve data quality.

仪器：

Thermo Scientific TSQ Quantum mass spectrometer

结论：

　　The TurboFlow method described here has been developed and validated to industry recommended guidelines for clinical laboratories.

　　Isobaric interferences observed with a 1D LC-MS/MS method at low 25-OH D3 metabolite concentrations were much reduced by using a 2D-LC-MS/MS approach, and even further improved by using TurboFlow technology. The Transcend™ TLX-1 LC-MS/MS with TurboFlow technology improved the sensitivity and the signal-to-noise ratio.