The CCS-aware SpatialOMx® workflow opens new dimensions by combining the molecular and spatial information measuredby MALDI-TIMS Imaging with highly confident 4D-Omics annotations.MetaboScape® 2021b and SCiLS™ Lab 2021a provide the interface to match data from both ionization techniques and enable automatic and CCS-aware annotations of MALDI Imaging data. The CCS-value is a key component of this workflow.