| 应用 |
用于检测β-半乳糖苷酶(β-galactosidase)活性，是β-半乳糖苷酶的显色底物。经β-半乳糖苷酶催化后可产生黄色产物，在410－420nm波长可以检测吸光度。 |
| 产品介绍 |
溶于水、甲醇，水中溶解度可达15mg/ml。有吸湿性。 |
| 生化机理 |
β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period. |
| 别名 |
ONPG;邻硝基苯-β-D-吡喃半乳糖苷;o-Nitrophenyl β-D-galactopyranoside
ONPG |
