Camera | Manufacturer PCOChip type Front illuminated sCMOSChip size: 2560 × 2160 pixels* (Useable area limited to 1024 x 1024 for SIM imaging)Pixel size: 6.5 µmReadout speeds: 95 MHz, 286 MhzReadout modes: Rolling shutter, global shutterCamera interface: Camera-linkBit depth: 15 bitQuantum efficiency: ~ 60%Dynamic range: 1:15 000Read noise: 1.5 e at 33 fps, 2 e at 100 fpsOn-chip binning: Not supported |
Focus | Laser based hardware autofocusCoarse/Long travel focus- Nanomover stepper motorFine/High speed focus - Piezo actuator |
Magnification | Super-resolution mode requires 60x to support camera configuration.Optional magnifications for widefield and TIRF imaging modes. |
Plate Positioning (XY Stage) | Stage range (xy): 24 × 48 mmy flatness: < 1 µm/mm*x flatness: < 1 µm/mm*xyz Nanomover repeatability: 10 µm/25 mm travelz piezo step resolution: 5 nmxyz Nanomover step resolution: 100 nmSample holder types Standard: 75 × 25 mm slides, 35 mm dishes, Multichamber coverslips |
Filter Selection | Standard filters for DAPICFPFITC/AF488YFPAF568/Texas RedCy5/AF647 |
Plate Compatability | Not Applicable |
Computer | OMX Master Workstation CPU Intel Core™ i7 processorClock speed 3.4 GHzMemory 16 GB DDR3 1333 MHz ECCPower supply 500 WDrives 1 TB SATA boot driveRAID 3 × 1 TB SATA in RAID5 configurationVideo card NVidia™ NVS300 VRAM 512 MBVideo bus PCIEMonitor panel size 24 in (60.96 mm) viewable areaResolution 1920 × 1200Operating system CentOS 6 or laterSpecifications subject to change without notice |
Height | 1575 mm |
Width | 864 mm |
Depth | 991 mm |
Weight | Approximately 480 kg |
Objectives | Standard Lenses60x 1.42 NA Oil Immersion PSF for SIM Imaging60x 1.49 NA TIRF for TIRF imagingOptional objectives20x/ 0.75 Super-Plan APO40x/ 1.35 Oil Plan APO60x/ 1.3 Silicone Plan APO100x/ 1.4 Oil Super-Plan APO100x/ 1.49 TIRF APO |
Optical System | 2 Illumination input paths for 3D-SIM/2D-SIM/2D-SIM-TIRF/Widefield and, Ring-TIRF/PK/Widefield modesUp to 4 camera detection channels |
Slide Compatibility | Standard microscope slides35 mm glass bottom dishesMultiwell chambered coverslips |
Software | OMX Master Control Software - AcquireSRsoftWoRx reconstruction and analysis software |
Light Source | Multicolor laser illumination |
Imaging Modes | Fluorescence imaging with deconvolutionDIC imaging3D-SIM super-resolution2D-SIM super-resolution2D-SIM TIRF super-resolutionLocalization microscopy super-resolution (e.g. PALM, STORM) |
Image Acquisition Features | Multiple camera capabilitySuper-resolution imagingWidefield imagingDIC imaging3D sectioningTimelapse imagingCell trackingMultipoint imaging |
Optional Hardware | DeltaVision Localization Microscopy System (29-0876-63 )Environmental Control Module and Live-Cell Kit (29-1330-64)Universal Long Working Distance (LWD) Condenser Assembly with DIC Prism Holder Ring (29-1330-63)Offline SI Workstation (53-853053-000) |