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据Cytoskeleton的副总裁Ashley Davis介绍，GOBlot的一个主要优势是一抗（实验中zei昂贵的试剂）可以回收利用。科学家曾经做过实验，将一抗回收后再重复使用三次，结果都很好，信号损失可忽略不计。
Prasanna Satpute-Krishnan是GOBlot™的忠实粉丝。“在实验室的启动阶段，实验室只有我一个，GOBlot对我帮助很大，”她说。“我只需要花10-15分钟的时间来计划我的Western blot，并加入封闭液、一抗和二抗，以及洗涤液，之后就可以不受干扰地做其他事情，让机器来完成Western blot。我zei喜欢的功能是单独收集用过的一抗溶液，以便再次使用。总之，GOBlot不但节约了时间，也节省了经费。”
剑桥大学的研究助理Amy Emery在癌症研究中也定期开展Western blot，并且用的是定制合成的抗体。“尽管我明白，自动化处理设备可以节省时间，因为无需手动洗涤。不过我一直不敢用，因为在我的印象中，抗体会有明显的损失。一抗真的很贵，所以我不愿意接受不必要的浪费，但Western blot设备似乎有了很大的改进，既然能重用一抗，那么我愿意尝试一下，”她说。
The GOBlot™ Western Blot Processor is a reliable fluid delivery device for reproducibly probing blots and membranes with primary and secondary antibodies. It was developed after communication with hundreds of research scientists over the past two years. They required a cost-effective machine that would reproduce the hands-on method using a rocker platform and exchanging buffers manually.
The equipment is composed of valves, pumps, a motorized tilting platform and a control board that coordinates timing with the application of the correct solution to the membrane or blot. It can operate at room temperature or in a cold room*. All commonly used buffers are applicable, e.g. PBST and TBST. The purchase of the machine comes with a full warranty (parts & labor or replacement) for up to one year after the date of purchase.
* - Note cold room operation on order if applicable.
The GOBlot™ allows the operator to load up of their own membranes, add their blocking solution, primary and secondary antibodies. Plus, the GOBlot makes it easy to recycle your primary antibody.
Choose from the four pre-loaded routines (See Manual). Then press the start button and walk away. Return after four hours to develop your blot using fluorescence, chemiluminescence or another method of detection.
The machine is designed to be modular and economical so that you utilize multiple machines running at different times with different routines and different antibodies, in other words, it’s very flexible for multiple users. Also being modular means that you can add new units when the budget allows or your project demands it.
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