You will need the following reagents: 5x Sample Buffer 10% w/v SDS 10 mM Dithiothreitol, or beta-mercapto-ethanol 20 % v/v Glycerol 0
NuPAGE GelsA gel electrophoresis system used for SDS-PAGE protein analysis
References: Watterson et al
ELECTROPHORESIS OF DNA IN POLYACRYLAMIDE GELSGel SizesSmall: 165 x 130 mmMedium: 165 x 200 mmLarge: 165 x 260 mm5% Analytical GelsReagent1 mm Small1 mm Medium1 mm Large10X TBE Buffer (ml)33
Direct Enzymatic Digestion of Protein Complexes Sherry Niessen, Ian McLeod and John R
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