质粒的大量制备

·         Plasmid Mini and Maxi Prep Methods (Gimila Lab) 

·         Maxi-preps and all media, solutions (NWFSC)
Isolation of cosmid, plasmid and P1 DNAs via diatomaceous earth-based Maxi, Midi and Mini-preps, and the non-diatomaceous earth-based Mini-prep.

• Large Scale Plasmid Preparation (Donis Keller Lab)
  For recovery of highly purified recombinant plasmid DNA using a CsCl2 gradient.
    

·         Plasmid Maxiprep (Bowtell Lab)

·         Large scale double-stranded DNA isolation (Roe Lab)
Provides protocol of isolating double-stranded DNA from plasmid or cosmid by the alkaline lysis procedure followed by equilibrium ultracentrifugation in cesium chloride-ethidium bromide gradients and diatomaceous earth-based purification method 

·         Large Scale Plasmid, Cosmid, BAC, PAC, and Fosmid DNA Isolation by a Cleared Lysate Method Followed by Double Acetate Precipitation (Roe Lab)

·         CsCl Prep. of Plasmid DNA (Mike A. Dyer)
This is a standard large scale prep. for plasmid DNA which gives a yield of 0.5 - 1.0 mg.