Using sterile pipette tips, transfer a 1 mm colony into 50 uL of 60 U/ml Zymolyase
3 uL of 1 U/mL Zymolyase stock solution
47 uL of water
Incubate cell suspension at 37 C for 30 minutes.
Incubate cell suspension at 95 C for 10 minutes.
Use 5 uL of the cell suspension for a 50 uL PCR reaction
Zymolase Stock Solution
10,000 U Zymolyase (Sigma)
1 mL KHPO4 buffer, pH 7.5 (adjusted with KOH)
0.2 mL 5M NaCl
5 mL 100% glycerol
Add H2O to 10 mL
Store at -20 C
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