10.1.  Columns used in chromatography should be appropriate for their intended use.

色谱所用柱子应适合其既定用途。

10.2. Columns should be purchased from approved suppliers.

色谱柱应从批准的供应商处采购。

10.3. Columns should be verified on receiptand checked for their suitability prior to use.

收到色谱柱时应验收，使用前应检查其是否适用性。

10.4. Columns, tubing and fittings should be appropriate to ensure that the system performs as expected.

色谱柱、管和配件应适当，以确保系统性能达到预期。

10.5. Backpressure and flow rates should be appropriate for the column to be used and specified in specifications and test procedures.

背压和流速应适合所用色谱柱，并在标准和检验方法中指定。

10.6. Column efficiency should be appropriate (number of theoretical plates) to ensure good chromatography.

柱效应适当（理论塔板数）以确保良好的色谱效果。

10.7. Risks associated with columns, such as“chiral columns”, should be controlled.

色谱柱相关风险，如“手性柱”应受控。

10.8. Flow rate, loading capacity and backpressure should be appropriate to ensure desired and constant retention time.

流速度、载量和背压应适当，以确保获得理想稳定的保留时间。

10.9. The use of columns should be recorded in a traceable manner. This includes, for example, a unique identification number, number of injections and washing.

色谱柱的使用应以可追踪的方式记录。其中包括例如唯一识别号、进针数和冲洗。

10.10. Columns should be washed (cleaned orflushed) according to defined procedures which define the steps and parameters, such as sequence and flow rate.

色谱柱应根据指定的程序清洗（清洁或冲洗），在程序中规定冲洗步骤和参数如顺序和流速。

10.11. Columns should be stored in a manner that ensures that they are not damaged.

色谱柱存贮方式应确保其不受损坏。

10.12. Equilibrating columns before, and temperature control of column and mobile phase during analysis, should be done when specified.

分析前柱平衡、分析中柱温控制和流动相如有规定应遵守。

10.13. Removal of contaminants and regeneration of columns should only be considered when the appropriate procedures for this had been developed.

只有在已制订了适当程序时，才可考虑清除色谱柱污染物和再生。